Epidemiologic data
in the last years have confirmed Human papillomavirus (HPV) as an
indispensable factor in cervical carcinogenesis. New diagnostic techniques
have been developed since in the prevention of cervical cancer. The
HPV group is dedicated to:
evaluation of new technologies for screening and diagnosis
of HPV related cancers
introduction of HPV detection and typing in the management of patients
with
cytological abnormalities detected in screening settings
research projects in cervical cancer and skin carcinogenesis (see
below)
Presently the project that involves more
investigators is a retrospective international survey of HPV types
in cervical cancer. The aim of this study is to collect data on the
distribution of the different HPV types in a long period of time (from
1950 until now) and to enlarge geographic distribution knowledge of
HPV type involvement in invasive cervical cancer. Histopathology is
performed prior to molecular techniques. Amplification of a short fragment
corresponding to the L1 gene of HPV, followed by reverse hybridization
techniques for specific typing are used. Variant analyses of HPV 16
and 18 will be performed in a subset of cases. This project is done
in collaboration with the Department of Epidemiology and Cancer Registry
of the same institution. SOPs have been established in the performance
of our HPV team. This project is partially funded by FIS.
The second research project focuses in the detection of HPV in skin lesions from
renal transplant recipients. In collaboration with the Vrije Universiteit of
Amsterdam a new assay has been developed to amplify and type HPV, genus beta
and gamma (HPV skin types). Malignant, premalignant and benign lesions from patients
are analyzed. Normal skin is collected by scraping different areas (sun exposed
and non-exposed) to establish the role of HPV as a possible risk factor in skin
carcinogenesis of immunosuppressed patients. The clinical part of this project
is done in the Dermatology Department, Hospital Germans Trias i Pujol and is
also funded by FIS.